Comparison of Two-Dimensional Fractionation Techniques for Shotgun Proteomics
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چکیده
منابع مشابه
comparison of the amount of debris extruded apically in two rotary techniques: flexmaster and m2
چکیده ندارد.
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Efficient fractionation of peptides is an essential prerequisite for comprehensive analysis of complex protein mixtures by shotgun mass spectrometry. The separation of peptides by isoelectric focusing is particularly attractive due to its orthogonality to reverse-phase HPLC. Here, we present a protocol for in-gel peptide isoelectric focusing using immobilized pH gradient strips. The method show...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2008
ISSN: 0003-2700,1520-6882
DOI: 10.1021/ac8007994