Compartmentalized cross-linked enzymatic nano-aggregates (c-CLEnA) for efficient in-flow biocatalysis
نویسندگان
چکیده
منابع مشابه
Optimization of Enzymatic Synthesis of Ampicillin Using Cross-Linked Aggregates of Penicillin G Acylase
Penicillin G acylase from E. coli TA1 was immobilized by Cross-Linked Enzyme Aggregates (CLEA), a new method for immobilization. This biocatalyst and commercial immobilized penicillin G acylase (PGA-450) were used to study the effect of pH, temperature and substrate concentration on the synthesis of ampicillin from phenyl glycine methyl ester (PGME) and 6-aminopenicillanic acid (6-APA). Compare...
متن کاملOptimization of Enzymatic Synthesis of Ampicillin Using Cross-Linked Aggregates of Penicillin G Acylase
Penicillin G acylase from E. coli TA1 was immobilized by Cross-Linked Enzyme Aggregates (CLEA), a new method for immobilization. This biocatalyst and commercial immobilized penicillin G acylase (PGA-450) were used to study the effect of pH, temperature and substrate concentration on the synthesis of ampicillin from phenyl glycine methyl ester (PGME) and 6-aminopenicillanic acid (6-APA). Compare...
متن کاملoptimization of enzymatic synthesis of ampicillin using cross-linked aggregates of penicillin g acylase
penicillin g acylase from e. coli ta1 was immobilized by cross-linked enzyme aggregates (clea), a new method for immobilization. this biocatalyst and commercial immobilized penicillin g acylase (pga-450) were used to study the effect of ph, temperature and substrate concentration on the synthesis of ampicillin from phenyl glycine methyl ester (pgme) and 6-aminopenicillanic acid (6-apa). compare...
متن کاملUse of Cross-Linked Enzyme Aggregates (CLEAs) for performing biotransformations
The methodology for the preparation of cross-linked enzyme aggregates (CLEAs) and their application as highly effective, recyclable biocatalysts in a wide variety of transformations is reviewed. The method constitutes a combination of enzyme purification and immobilization into a single operation and allows for the use of relatively crude enzyme preparations. The resulting CLEAs have excellent ...
متن کاملCross-linked sulfonated polyacrylamide (Cross-PAA-SO3H) attached to nano-Fe3O4 as a superior catalyst for the synthesis of oxindoles
Cross-linked sulfonated polyacrylamide (Cross-PAA-SO3H) attached to nano-Fe3O4 as an efficientheterogeneous solid acid catalyst has been used for the preparation of spiro[pyrazoloquinoline-oxindoles]and spiro[chromenopyrazolo-oxindoles] through a four-component reactions of phenylhydrazineor hydrazine hydrate, isatins, ketoesters and naphthylamine or 2-naphthol under reflux co...
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ژورنال
عنوان ژورنال: Chemical Science
سال: 2020
ISSN: 2041-6520,2041-6539
DOI: 10.1039/c9sc05420k