Complete sequence of a chicken lambda light chain immunoglobulin derived from the nucleotide sequence of its mRNA.
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چکیده
منابع مشابه
Nucleotide sequence of constant and 3' untranslated regions of a kappa immunoglobulin light chain mRNA of a homozygous b4 rabbit.
A homozygous a2/a2 and b4/b4 rabbit has been hyperimmunized with Micrococcus lysodeikticus. Poly(A)-containing RNA has been isolated from the spleen and translated in vitro, and translation products have been analyzed by NaDodSO4/polyacrylamide gel electrophoresis. Double-stranded cDNA has been synthesized from poly(A)-containing RNA template and inserted in the Pst I endonuclease site of plasm...
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Name of Sequence. Chicken prolactin (PRL) gene. Genus and Species. Gallus gallus (chicken). Origin of Clones. Genomic DNA from Xing Hua Chinese native chickens was used as a template. Primers were designed based on exon/intron junctions of the genomic turkey PRL gene sequence (Kurima et al., 1995). Polymerase chain reaction (PCR) amplifications of the PRL gene were performed by using the follow...
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The complete nucleotide sequence of the 957-kb DNA of the human immunoglobulin heavy chain variable (VH) region locus was determined and 43 novel VH segments were identified. The region contains 123 VH segments classifiable into seven different families, of which 79 are pseudogenes. Of the 44 VH segments with an open reading frame, 39 are expressed as heavy chain proteins and 1 as mRNA, while t...
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We have previously published the sequence of the 5' end of the yeast MY01 gene (Watts et aL, 1987). This gene was isolated from a X47.1 genomic library screened with a probe containing the active thiol region of the nematode myosin UNC54 (Karn et ai., 1983). Here we present its complete nucleotide sequence achieved by the dideoxy chain termination method (Sanger, 1977). Templates cloned in pUC1...
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We have determined the sequence of the mRNA encoding cytosolic phosphoenolpyruvate carboxykinase (GTP) [GTP:oxaloacetate carboxy-lyase (transphosphorylating), EC 4.1.1.32] from the chicken and have deduced the primary structure of the protein. The message for the enzyme is 2762 bases long and encodes a protein of 622 amino acids with a molecular mass of 69,522 daltons. The 5' untranslated regio...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1983
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.80.13.4099