Considerations towards a roadmap for collection, handling and storage of blood extracellular vesicles
نویسندگان
چکیده
منابع مشابه
Handling and storage of human body fluids for analysis of extracellular vesicles.
Because procedures of handling and storage of body fluids affect numbers and composition of extracellular vesicles (EVs), standardization is important to ensure reliable and comparable measurements of EVs in a clinical environment. We aimed to develop standard protocols for handling and storage of human body fluids for EV analysis. Conditions such as centrifugation, single freeze-thaw cycle, ef...
متن کاملEXTRACELLULAR VESICLES AND THEIR THERAPEUTIC APPLICATIONS: A REVIEW
Background & Aims: Extracellular vesicles are double phospholipid layer vesicles releasing from several cells. These particles contain bio-molecules including mRNA, miRNA, DNA, proteins, and lipids which play a pivotal role in the cell to cell communication. Once delivered, they participate to change target cell function, fate, morphology, differentiation, and growth. The present study is a re...
متن کاملConsiderations for Applying Metabolomics to the Analysis of Extracellular Vesicles
Cell-derived extracellular vesicles (EVs) are involved in the development of different pathologies including inflammatory diseases and cancer (1) and have demonstrated a promising potential for human diagnostic (2) and therapeutic applications (3). Constitutive secretion of these vesicles has been described for platelets, reticulocytes, dendritic cells, lymphocytes, mast cells, intestinal epith...
متن کاملTowards traceable size determination of extracellular vesicles
BACKGROUND Extracellular vesicles (EVs) have clinical importance due to their roles in a wide range of biological processes. The detection and characterization of EVs are challenging because of their small size, low refractive index, and heterogeneity. METHODS In this manuscript, the size distribution of an erythrocyte-derived EV sample is determined using state-of-the-art techniques such as ...
متن کاملIsolation and characterization of Faecalibacterium prausnitzii extracellular vesicles
Introduction: Extracellular vesicles (EVs) contain active biological compounds which play important roles in biological processes. The secretion of EVs is a common phenomenon which occurs in archaea, bacteria and mammalian cells. The secretion of bacterial EVs has been discovered in various species of both Gram-negative and Gram-positive bacteria. Faecalibacterium prausnitzii is one of the comm...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Extracellular Vesicles
سال: 2019
ISSN: 2001-3078
DOI: 10.1080/20013078.2019.1647027