Construction of a cDNA Encoding a Repetitive Amino Acid Sequence
نویسندگان
چکیده
منابع مشابه
Strategy for construction of a cDNA encoding a repetitive amino acid sequence.
PCR is a powerful method with which to generate almost any desired cDNA sequence. Construction of cDNA fragments that encode repetitive amino acid sequences by PCR has proven problematic, however, because repetitive nucleotide primers tend to amplify undesired fragments because of their mis-annealing with the template. To overcome this problem, Dombrowski and Wright (2) developed a solid-phase ...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2000
ISSN: 0736-6205,1940-9818
DOI: 10.2144/00295bm10