Construction of cDNA library and preliminary analysis of expressed sequence tags from Siberian tiger
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چکیده
منابع مشابه
Construction of cDNA library and preliminary analysis of expressed sequence tags from Siberian tiger
In this study we successfully constructed a full-length cDNA library from Siberian tiger, Panthera tigris altaica, the most well-known wild Animal. Total RNA was extracted from cultured Siberian tiger fibroblasts in vitro. The titers of primary and amplified libraries were 1.30×10(6) pfu/ml and 1.62×10(9) pfu/ml respectively. The proportion of recombinants from unamplified library was 90.5% and...
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ژورنال
عنوان ژورنال: International Journal of Biological Sciences
سال: 2010
ISSN: 1449-2288
DOI: 10.7150/ijbs.6.584