Context affects nuclear protein localization in Saccharomyces cerevisiae.

the significance of c-terminal nls sequences of vird2 in its nuclear localization in saccharomyces cerevisiae

agrobacterium tumefaciens is capable of gene transfer to both plant and non-plant organisms. indeed, upon infection of eukaryotic cells, agrobacterium tumefaciens transfers a piece of its tumor inducing (ti)-plasmid, called t-dna, to the host cell nucleus, which subsequently integrates into the host genome. the vird2 virulence protein which has relaxase endonuclease activities covalently binds ...

Gln3p nuclear localization and interaction with Ure2p in Saccharomyces cerevisiae.

Gln3p is one of two well characterized GATA family transcriptional activation factors whose function is regulated by the nitrogen supply of the cell. When nitrogen is limiting, Gln3p and Gat1p are concentrated in the nucleus where they bind GATA sequences upstream of nitrogen catabolite repression (NCR)-sensitive genes and activate their transcription. Conversely, in excess nitrogen, these GATA...

Localization of nuclear retained mRNAs in Saccharomyces cerevisiae.

In the yeast Saccharomyces cerevisiae, a common conditional phenotype associated with deletion or mutation of genes encoding mRNA export factors is the rapid accumulation of mRNAs in intranuclear foci, suggested to be near transcription sites. The nuclear RNA exosome has been implicated in retaining RNAs in these foci; on deletion of the exosome component Rrp6p, the RNA is released. To determin...

Nuclear localization of aspartate transcabamoylase in Saccharomyces cerevisiae

The cytochemical technique using the in situ precipitation of orthophosphate ions liberated specifically by the aspartate carbamoyltransferase (ATCase) (EC 2.1.3.2) reaction indicated that in Saccharomyces cerevisiae this enzyme is confined to the nucleus. This observation is in accordance with the result reported by Bernhardt and Davis (1972), Proc. Natl. Acad. Sci. U. S. A. 69:1868-1872) on N...

Localization and Mutational Analysis of the Nuclear and Aggregation-Prone Ime4 Protein in Saccharomyces cerevisiae