Controllable Ion Channel Expression through Inducible Transient Transfection

Ion channel activity drives ion channel expression.

Ion permeation through neuronal nAChR ion channel

Alzheimer’s dementia is related to loss of inter-neuron communication by nicotinic acetylcholine receptors (nAChR) at post synaptic neurons, and successful therapy approaches rely e.g. on modulations of response signals initiated by ion flux through the receptor integrated ion channel. We have evaluated Umbrella Sampling (US) and Steered Molecular Dynamics (SMD) pulling for calculation of the p...

Astrovirus ribosomal frameshifting in an infection-transfection transient expression system.

Different regions of the human astrovirus frameshift signal were cloned into the rhesus rotavirus VP4 gene and evaluated in an infection-transfection transient expression cell culture system. BHK-21 cells, infected with a vaccinia virus that expresses T7 RNA polymerase (vTF7-3), were transfected with the various astrovirus-VP4 constructs. All constructs were driven by a T7 promoter and containe...

Transient transfection and expression of firefly luciferase in Giardia lamblia.

We have developed a gene transfer system for the protozoan parasite Giardia lamblia. This organism is responsible for many cases of diarrhea worldwide and is considered to be one of the most primitive eukaryotes. Expression of a heterologous gene was detected in this parasite after electroporation with appropriate DNA constructs. We constructed a series of transfection plasmids using flanking s...

Regulation of ion channel expression.

A potentially important mechanism controlling ion channel expression is homeostatic regulation, which can act to maintain a stable electrophysiological phenotype in cardiac myocytes as well as to provide plasticity in response to genetic, pathological, or pharmacological insults. The capabilities and limitations of the homeostatic regulatory mechanisms that contribute to the control of cardiac ...