Controlling Redox Status for Stem Cell Survival, Expansion, and Differentiation
نویسندگان
چکیده
منابع مشابه
Controlling Redox Status for Stem Cell Survival, Expansion, and Differentiation
Reactive oxygen species (ROS) have long been considered as pathological agents inducing apoptosis under adverse culture conditions. However, recent findings have challenged this dogma and physiological levels of ROS are now considered as secondary messengers, mediating numerous cellular functions in stem cells. Stem cells represent important tools for tissue engineering, drug screening, and dis...
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Successful isolation, derivation and culturing of human pluripotent stem cells, including human embryonic stem cells (hESCs) and human induced pluripotent stem (hiPSCs) cells in laboratory scale has opened new horizones for cell therapy applications such as tissue engineering and regenerative medicine. However, most of the cell therapy protocols using these unique cells require large number of ...
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BACKGROUND Human stem cells are cellular resources with outstanding potential for cell therapy. However, for the fulfillment of this application, major challenges remain to be met. Of paramount importance is the development of robust systems for in vitro stem cell expansion and differentiation. In this work, we successfully developed an efficient scalable bioprocess for the fast production of h...
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from OXPHOS to glycolysis [6]. The pluripotent marker OCT4 affects several target genes (e.g. ATP5D) associated with energy metabolism which are in favor of glycolysis [1]. Various new hPSC culture systems have been developed in recent years, including defined serum-free media (e.g., mTESR, StemPro, and E8) and synthetic substrates (e.g., synthemax) [7,8]. How the subtle differences in these cu...
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Introduction In vitro expansion and differentiation of mesenchymal stem cells (MSCs) rely on specific environmental conditions. One crucial factor is the oxygen tension. Low oxygen tension improves MSC expansion in vitro. Moreover, it accelerates the induction of a chondrogenic phenotype in subsequent 3D differentiation cultures. However, clones of MSCs from the same donor often differ in their...
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ژورنال
عنوان ژورنال: Oxidative Medicine and Cellular Longevity
سال: 2015
ISSN: 1942-0900,1942-0994
DOI: 10.1155/2015/105135