Conversion of AFLP markers to sequence-specific PCR markers in barley and wheat
نویسندگان
چکیده
منابع مشابه
Expressed sequence tag-PCR markers for identification of alien barley chromosome 2H in wheat.
We developed EST-PCR markers specific to barley chromosome 2H, for the purpose of effectively tracing alien chromosomes or chromosome parts in the wheat genetic background. The target alien chromosome 2H confers high resistance to pre-harvest sprouting, which is a worldwide natural disaster in wheat. A total of 120 primer pairs were selected by combining the wheat group 2 chromosomes of th...
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the present study aimed to investigate representation of discourse markers and metadiscourse markers in conversations and readings of general elt coursebook series used in the language centers of iran. to this aim, four elt coursebooks popularly taught in language centers of this country were analyzed based on fung and carter’s (2007) framework regarding discourse markers and hyland’s (2005) fr...
Identification of Male-Specific AFLP Markers in Dioecious Texas Bluegrass
is more common among the dicots than the monocots (Renner and Ricklefs, 1995). Dioecy is a breeding system that promotes cross-pollination in Poa is a large, diverse genus that belongs to the subplants. The transfer of this trait into economically important selfpollinated cereal crops would revolutionize the production of hybrids family Pooideae of the Poaceae. Several species are in these spec...
متن کاملStudy of Genetic Variation in Wild Diploid Wheat (Triticum boeoticum) from Iran Using AFLP Markers
Little information is available regarding genetic variation in wild wheat relatives from Iran. In this study, genetic diversity of 36 populations of wild einkorn wheat, Triticum boeoticum, was studied using amplified fragment length polymorphism (AFLP) primer combinations. Seventeen AFLP primer combinations led to amplify 979 scorable fragments ranging from 50 to 500 bp and of these, 429 (44%) ...
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Molecular genetic research relies heavily on the ability to detect polymorphisms in DNA. Single nucleotide polymorphisms (SNPs) are the most frequent form of DNA variation in the genome. In combination with a PCR assay, the corresponding SNP can be analyzed as a derived cleaved amplified polymorphic sequence (dCAPS) marker. The dCAPS method exploits the well-known specificity of a restriction e...
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ژورنال
عنوان ژورنال: Theoretical and Applied Genetics
سال: 1999
ISSN: 0040-5752,1432-2242
DOI: 10.1007/s001220051169