Correction: Antisense precision polymer micelles require less poly(ethylenimine) for efficient gene knockdown

Modified Polyethylenimine: Self Assemble Nanoparticle Forming Polymer for pDNA Delivery

Objective Polyethylenimine (PEI), a readily available synthetic polycation which has high transfection efficiency owing to its buffering capacity was introduced for transfection a few years ago. But it has been reported that PEI is cytotoxic in many cell lines. In this study, in order to enhance the transfection efficiency of 10 kDa PEI and reduce its toxicity, hydrophobic residues were grafte...

Antisense Knockdown of Sphingosine

Gene knockdown in Paracoccidioides brasiliensis using antisense RNA.

Paracoccidioides brasiliensis is a thermal dimorphic fungus which in the host environment exhibits a multinucleated and multibudding yeast form. The cellular and molecular mechanisms underlying these phenotypes remain to be clarified, mostly due to the absence of efficient classical genetic and molecular techniques. Here we describe a method for gene expression knockdown in P. brasiliensis by a...

Polymer Micelles for Drug Delivery

Polymeric micelles obtained from the self-assembly of amphiphilic block copolymers are probably one of the most common drug delivery carriers among polymeric nanoparticles.1–4 The rise of highly controlled polymerization techniques, especially processes such as ATRP5 and RAFT,6 has led to an extraordinary surge of new types of block copolymers fit for biomedical applications. Facile control ove...

A Polyethylenimine-Linoleic Acid Conjugate for Antisense Oligonucleotide Delivery

A novel antisense oligonucleotide (ASO) carrier, polyethylenimine conjugated to linoleic acid (PEI-LA), was synthesized and evaluated for delivery of LOR-2501 to tumor cells. LOR-2501 is an ASO targeting ribonucleotide reductase R1 subunit (RRM1). In this study, PEI-LA was synthesized by reacting PEI (Mw ~ 800) with linoleoyl chloride. Gel retardation assay showed complete complexation between ...