Cryopreservation of orchid seeds through rapid and step freezing methods

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Cryopreservation of orchid seeds through rapid and step

Ecuador has a great variety of climatic regions that potentiate biodiversity. The family constitutes one of the most important of the country, having Orchidaceae identified about 4032 species with a high degree of endemism, therefore the development and research of alternative methods of storage and conservation of species is a strategy of primary interest for researchers and for society in gen...

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Precision-cut tissue slices of both hepatic and extra-hepatic origin are extensively used as an in vitro model to predict in vivo drug metabolism and toxicity. Cryopreservation would greatly facilitate their use. In the present study, we aimed to improve (1) rapid freezing and warming (200 degrees C/min) using 18% Me(2)SO as cryoprotectant and (2) vitrification with high molarity mixtures of cr...

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ژورنال

عنوان ژورنال: F1000Research

سال: 2018

ISSN: 2046-1402

DOI: 10.12688/f1000research.13622.1