Cryopreserved neonatal hepatocytes may be a source for transplantation: Evaluation of functionality toward clinical use

[The clinical use of cryopreserved human skin allografts for transplantation].

BACKGROUND The biological recovery of human skin allografts is the gold standard for preservation in Skin Banks. However, there is no worldwide consensus about specific allocation criteria for preserved human skin allografts with living cells. A report is presented on the results of 5 years of experience of using human skin allografts in burned patient in the Skin and Tissue Bank at the "Instit...

AdipoRon may be benefit for atherosclerosis prevention

Atherosclerosis has serious role in coronary arteries disease,so it is important to establish effective strategies for prevention or even treatment of atherosclerosis. Adiponectin, as one of the most abundant adipokines, has insulin sensitivity, anti-inflammatory and anti-atherogenic properties. Disturbed adiponectin actions through its receptor, (AdipoR1 and AdipoR2) may be involved in atheros...

A novel in vitro experimental system for the evaluation of drug metabolism: Cofactor-supplemented permeabilized cryopreserved human hepatocytes (MetMax™ Cryopreserved Human Hepatocytes).

We report here a novel experimental system - MetMax™ cryopreserved human hepatocytes (MMHH), for in vitro drug metabolism studies. MMHH consist of cofactor-supplemented permeabilized cryopreserved human hepatocytes. The use procedures for MMHH are significantly simplified from that for conventional cryopreserved human hepatocytes (CCHH): 1. Storage at -80o C instead of in liquid nitrogen; 2. Us...

A novel in vitro experimental system for the evaluation of drug metabolism: Cofactor-supplemented permeabilized cryopreserved human hepatocytes (MetMaxTM Cryopreserved Human Hepatocytes)

Comparison of cryopreserved HepaRG cells with cryopreserved human hepatocytes for prediction of clearance for 26 drugs.

Prediction of clearance in drug discovery currently relies on human primary hepatocytes, which can vary widely in drug-metabolizing enzyme activity. Potential alternative in vitro models include the HepaRG cell (from immortalized hepatoma cells), which in culture can express drug-metabolizing enzymes to an extent comparable to that of primary hepatocytes. Utility of the HepaRG cell will depend ...