Crystal structure ofN-formimino-L-glutamate iminohydrolase

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Structure of N-Formimino-l-glutamate Iminohydrolase from Pseudomonas aeruginosa

N-Formimino-l-glutamate iminohydrolase (HutF), from Pseudomonas aeruginosa with a locus tag of Pa5106 ( gi|15600299 ), is a member of the amidohydrolase superfamily. This enzyme catalyzes the deamination of N-formimino-l-glutamate to N-formyl-l-glutamate and ammonia in the histidine degradation pathway. The crystal structure of Pa5106 was determined in the presence of the inhibitors N-formimino...

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Mechanistic characterization of N-formimino-L-glutamate iminohydrolase from Pseudomonas aeruginosa.

N-Formimino-l-glutamate iminohydrolase (HutF) from Pseudomonas aeruginosa catalyzes the deimination of N-formimino-l-glutamate in the histidine degradation pathway. An amino acid sequence alignment between HutF and members of the amidohydrolase superfamily containing mononuclear metal centers indicated that residues Glu-235, His-269, and Asp-320 are involved in substrate binding and activation ...

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N-formimino-L-glutamate formiminohydrolase of Aerobacter aerogenes.

N-Formimino-n-glutamate is an intermediate in the degradation of n-histidine by such different types of cells as those of animal liver (1)) Pseudomonas jluorescens (2, 3), and Aerobacter aerogenes (3). The pathway leading from histidine to formiminoglutamate is the same in all cases and comprises three steps catalyzed by distinct enzymes: L-histidine is converted to urocanate and ammonia by his...

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In the title compound (alternative name N-carboxy-l-glutamic anhydride γ-ethyl ester), C8H11NO5, the oxazolidine ring is essentially planar, with a maximum deviation of 0.019 (2) Å. In the crystal, mol-ecules are linked by N-H⋯O hydrogen bonds between the imino group and the carbonyl O atom in the ethyl ester group, forming a tape structure along the c-axis direction. The oxazolidine rings of a...

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Crystal structure of γ-methyl l-glutamate N-carb­oxy anhydride

In the title compound, C7H9NO5, alternative name N-carb-oxy-l-glutamic anhydride γ-methyl ester, the oxazolidine ring is essentially planar with a maximum deviation of 0.020 (3) Å. In the crystal, mol-ecules are linked by N-H⋯O hydrogen bonds between the imino group and the carbonyl O atom in the methyl ester group, forming a tape structure along the a-axis direction. The tapes are linked by C-...

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ژورنال

عنوان ژورنال: Acta Crystallographica Section A Foundations of Crystallography

سال: 2010

ISSN: 0108-7673

DOI: 10.1107/s0108767310096807