Cultivation of M. leprae in Cell-Free, Semi-Synthetic Softagar Media

Cultivation and Viability Determination of Mycobacterium leprae

Mycobacterium leprae, despite being recognized as a human pathogen over 140 years ago, remains uncultivable in microbiological culture media or in cell culture systems. Although it is now well established that M. leprae prefers cooler temperatures, slightly acidic microaerophilic conditions, and lipids rather than sugars as an energy source, the exact parameters for a defined axenic medium that...

Attempts at cultivation of Mycobacterium leprae in cell culture under regulation of redox potential at environment.

determination of maximal singularity free zones in the workspace of parallel manipulator

due to the limiting workspace of parallel manipulator and regarding to finding the trajectory planning of singularity free at workspace is difficult, so finding a best solution that can develop a technique to determine the singularity-free zones in the workspace of parallel manipulators is highly important. in this thesis a simple and new technique are presented to determine the maximal singula...

Schwann cell invasion by M. leprae: the probable trojan horse.

The ability of Mycobacterium leprae to invade and sustain inside Schwann cells is vital in the pathogenesis of leprosy. M. leprae has the capacity to induce Schwann cell proliferation thereby providing enough host cells for bacterial propagation. It also has the ability to cause extensive demyelination of peripheral nerves, the cause for most of its dreaded sequelae. Whether this demyelination ...

Cultivation of Trypanosoma brucei sspp. in semi-defined and defined media.