CYPRIDINA LUCIFERIN. PARTITION CHROMATOGRAPHY
نویسندگان
چکیده
منابع مشابه
Stereoselective incorporation of isoleucine into Cypridina luciferin in Cypridina hilgendorfii (Vargula hilgendorfii).
The emission of light in the marine ostracod Cypridina hilgendorfii (presently Vargula hilgendorfii) is produced by the Cypridina luciferin-luciferase reaction in the presence of molecular oxygen. Cypridina luciferin has an asymmetric carbon derived from isoleucine, and the absolute configuration is identical to the C-3 position in L-isoleucine or D-alloisoleucine. To determine the stereoselect...
متن کاملStudies on the Amount of Light Emitted by Mixtures of Cypridina Luciferin and Luciferase
1. A photometric method was devised for measuring the intensities of light emitted per cc. of hiciferin solution and calculating the amount of light emitted per gm. of dried Cypridina powder. A total of 128 runs was made and the data are incorporated in this report. 2. The maximum amount of light emitted from 1 gm. of powder under the experimental conditions was 0.655 lumens. Different samples ...
متن کاملStudies on Bioluminescence. X. Carbon Dioxide Production during Luminescence of Cypridina Luciferin. by E. Newton Harvey
All luminous animals require oxygen for luminescence bu t it is not known whether they give off CO2 as a result of the luminescence. Luminous bacteria produce carbon dioxide but this is no doubt the carbon dioxide of respiration. Even if we could prove that luminous bacteria produced more carbon dioxide than certain non-luminous bacteria, this would not necessarily znean that the excess carbon ...
متن کاملEnhancement of fluoresceinyl cypridina luciferin analog chemiluminescence by human serum albumin for singlet oxygen detection.
Fluoresceinyl cypridina luciferin analog (FCLA) is a chemiluminescence (CL) probe for detecting reactive oxygen species (ROS). Its efficiency for detecting singlet oxygen (1O2) can be significantly enhanced in the presence of human serum albumin (HSA). This phenomenon may apply to important applications for both research and clinical testing, because of the broad presence of HSA in the human sy...
متن کاملAnion-exchange displacement centrifugal partition chromatography.
Ion-exchange displacement chromatography has been adapted to centrifugal partition chromatography. The use of an ionic liquid, benzalkonium chloride, as a strong anion-exchanger has proven to be efficient for the preparative separation of phenolic acid regioisomers. Multigram quantities of a mixture of three hydroxycinnamic acid isomers were separated using iodide as a displacer. The displaceme...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1952
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)55651-9