Cytochrome c modified by digestion with proteolytic enzymes: 1. Digestion

Cytochrome c modified by digestion with proteolytic enzymes. 2. Properties of pepsin-modified cytochrome c.

Modified by Digestion with Proteolytic Enzymes

Although the importance of prosthetic groups to the activity of certain enzymes has been much studied, the part played by the protein portion of the molecule has received little attention. Haemoglobin, catalase and peroxidase all have the same prosthetic group, namely, protohaematin, yet the attachment of different proteins gives them distinct and characteristic properties. Thus haemoglobin is ...

A Statistical Model of Proteolytic Digestion

We present a stochastic model of proteolytic digestion of a proteome, assuming the distribution of parent protein lengths in the proteome, the relative abundances of the 20 amino acids in the proteome, and the digestion “rules” of the enzyme used in the digestion. We derived a closed form expression for the fragment mass distribution for a large class of enzymes including the widely used Trypsi...

Peptic digestion of beef myofibrils is modified by prior marination

BACKGROUND Preparatory steps such as seasoning, marination, and cooking may induce changes in meat which affects the ability of the stomach to adequately digest it. This may result in peptide chains reaching the colon intact where resident bacteria ferment them resulting in the formation of putative carcinogenic phenolic by-products. OBJECTIVE In this study, we set out to determine whether pe...

Proteolytic digestion of Cl(-)-ATPase in rat brain synaptosomes.

Upon tryptic digestion of synaptosomes, ATPase activities decreased in the order of Cl(-)-ATPase greater than or equal to Na+,K+-ATPase greater than anion-insensitive Mg2+-ATPase. Upon synaptosome treatment with hypotonic solution, Cl(-)-ATPase or anion-insensitive Mg2+-ATPase was slightly inactivated, while Na+,K+-ATPase underwent a much larger degree of inactivation. ATP-Mg inhibited the ATPa...