DeepTracer for fast de novo cryo-EM protein structure modeling and special studies on CoV-related complexes
نویسندگان
چکیده
منابع مشابه
Modeling Protein Structure Features from Three Dimensional Cryo-EM Images
Secondary structure of protein, such as α-helix and βsheet, is the general three-dimensional (3D) form of local segments. It can be identified from the 3D electron cryomicroscopy (cryo-EM) density images at medium resolutions (~5-10Å). A detected β-sheet can be represented by either the voxels of β-sheet density or by many piecewise polygons to compose a rough surface. However, none of these is...
متن کاملTowards De Novo Folding of Protein Structures from Cryo-EM 3D Images at Medium Resolutions
Electron Cryo-microscopy (cryo-EM) is an important biophysical technique that produces 3dimensional (3D) images at different resolutions. De novo folding is becoming a promising approach to derive the backbone of proteins from the cryo-EM 3D images at medium resolutions. We summarize our findings in major steps of de novo folding and the challenges from the
متن کاملDe Novo Protein Structure Prediction
An unparalleled amount of sequence data is being made available from large-scale genome sequencing efforts. The data provide a shortcut to the determination of the function of a gene of interest, as long as there is an existing sequenced gene with similar sequence and of known function. This has spurred structural genomic initiatives with the goal of determining as many protein folds as possibl...
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Electron density maps of membrane proteins or large macromolecular complexes are frequently only determined at medium resolution between 4 Å and 10 Å, either by cryo-electron microscopy or X-ray crystallography. In these density maps, the general arrangement of secondary structure elements (SSEs) is revealed, whereas their directionality and connectivity remain elusive. We demonstrate that the ...
متن کاملCryo-EM structure of a transcribing cypovirus.
Double-stranded RNA viruses in the family Reoviridae are capable of transcribing and capping nascent mRNA within an icosahedral viral capsid that remains intact throughout repeated transcription cycles. However, how the highly coordinated mRNA transcription and capping process is facilitated by viral capsid proteins is still unknown. Cypovirus provides a good model system for studying the mRNA ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2020
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.2017525118