Dense sgRNA Library Construction Using a Molecular Chipper Approach
نویسندگان
چکیده
منابع مشابه
A Molecular Chipper technology for CRISPR sgRNA library generation and functional mapping of noncoding regions
Clustered regularly-interspaced palindromic repeats (CRISPR)-based genetic screens using single-guide-RNA (sgRNA) libraries have proven powerful to identify genetic regulators. Applying CRISPR screens to interrogate functional elements in noncoding regions requires generating sgRNA libraries that are densely covering, and ideally inexpensive, easy to implement and flexible for customization. He...
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ژورنال
عنوان ژورنال: BIO-PROTOCOL
سال: 2017
ISSN: 2331-8325
DOI: 10.21769/bioprotoc.2373