Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction
نویسندگان
چکیده
منابع مشابه
Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction.
We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5–6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, N...
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ژورنال
عنوان ژورنال: Eurosurveillance
سال: 2012
ISSN: 1560-7917
DOI: 10.2807/ese.17.39.20285-en