Detection of exocytosis at individual pancreatic beta cells by amperometry at a chemically modified microelectrode.
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چکیده
منابع مشابه
Detection of exocytosis at individual pancreatic beta cells by amperometry at a chemically modified microelectrode.
Amperometry at a carbon fiber microelectrode modified with a composite of ruthenium oxide and cyanoruthenate was used to monitor chemical secretions of single pancreatic beta cells from rats and humans. When the insulin secretagogues glucose, tolbutamide, and K+ were applied to the cell, a series of randomly occurring current spikes was observed. The current spikes were shown to be due to the d...
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Functional coupling between Ca(2+)-induced Ca(2+) release (CICR) and quantal exocytosis in 5-hydroxytryptamine-loaded INS-1 beta cells was assessed through the use of carbon fibre amperometry in combination with Fura-2. CICR was evoked by the glucagon-like-peptide-1 (GLP-1) receptor agonist exendin-4 (Ex-4) and was accompanied by quantal secretory events appearing as amperometric current spikes...
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This paper describes the first immunosensing system reported for the detection of bacteria combining immunomagnetic capture and amperometric detection in a one-step sandwich format, and in a microfluidic environment. Detection is based on the electrochemical monitoring of the activity of horseradish peroxidase (HRP), an enzyme label, through its catalysis of hydrogen peroxide (H(2)O(2)) in the ...
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The time course of extrusion of the vesicular contents during exocytosis has been examined at adrenal medullary cells with carbon-fiber microelectrodes. Two electrochemical techniques were used: cyclic voltammetry and amperometry. Spikes obtained by amperometry had a faster time course than those measured by cyclic voltammetry, consistent with the different concentration profiles established by...
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Caplllary zone electrophoresis (CZE) was employed to separate sugars accordlng to their electrophoretic moMlitks In strong alkallne clolutlons (pH ca. 13). Saccharide zones were monltored electrochemlcaliy udng amperometrlc detectlon at a constant potentlal, 0.6 V (vs Ag/AgCI), with a cyllndrlcal copper wire electrode (25 pm In diameter). The Cu-wire microelectrode In strong badc solutions had ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1995
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.92.21.9608