Detection of microsatellite polymorphisms without cloning

Detection of microsatellite polymorphisms without cloning.

Microsatellites or simple sequence repeats provide an attractive source of genetic polymorphisms for both mammals and plants (1). Microsatellites can be detected by PCR using minute amounts of starting material and they provide codominant markers with a high degree of allelic polymorphism. However, these markers are obtained through a difficult and labor-intensive procedure. In contrast, RAPD m...

Cloning of transfected cells without cloning rings.

was mutagenized by ethyl methanesulfate (EMS) according to Rose et al. (3) and plated to a density of 800 colonies/plate at 23°C for screening temperature-sensitive mutants. After 3 days, the colonies were replica-plated and grown at 23°C and 36°C. The temperature-sensitive strains were selected after 3 days by comparing the two plates according to the following procedure, which combines classi...

Growing Without Cloning

A graph G is claw-free if no induced subgraph of it is isomorphic to the complete bipartite graph K1,3, and it is prime if |V (G)| ≥ 4 and there is no X ⊆ V (G) with 1 < |X| < |V (G)| such that every vertex of V (G) \ X with a neighbour in X is adjacent to every vertex of X. In particular, if G is prime, then both G and Gc are connected. This paper has two main results. The first one is that if...

Quantum cloning without signaling

Perfect Quantum Cloning Machines (QCM) would allow to use quantum nonlocality for arbitrary fast signaling. However perfect QCM cannot exist. We derive a bound on the fidelity of QCM compatible with the no-signaling constraint. This bound equals the fidelity of the Bužek-Hillery QCM. Quantum mechanics is non-local, but this cannot be used to signal. Indeed, the different mixtures of pure states...

Cloning: a business without regulation.