Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene
نویسندگان
چکیده
منابع مشابه
Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene.
Synthetic oligonucleotide primers of 21 and 24 bases, respectively, were used in the polymerase chain reaction (PCR) to amplify a sequence of the nuc gene, which encodes the thermostable nuclease of Staphylococcus aureus. A DNA fragment of approximately 270 bp was amplified from lysed S. aureus cells or isolated DNA. The PCR product was detected by agarose gel electrophoresis or Southern blot a...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1992
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.30.7.1654-1660.1992