Detection of terpenoids and steroids in Lindsaea obtusa with thin layer chromatography

Abstract. Wutsqa YU, Suratman, Sari SA. 2021. Detection of terpenoids and steroids in Lindsaea obtusa with thin layer chromatography. Biofarmasi J Nat Prod Biochem 19: 65-69. The diversity ferns on this earth is very high, the potential benefits for human life are ornamental plants, food materials, medicines. not all known because lack information these ferns, one J. Sm. Ex Hook. phytochemical test first step research, searching new active compounds derived from natural ingredients. This study was conducted to determine differences terpenoid steroid profile L. extract using Thin Layer Chromatography (TLC). Extraction maceration techniques methanol n-hexane solvents. extraction results will be used screening TLC technique GF254 silica gel plate as a stationary phase, mobile phase chloroform: (9: 1 v/v), n-hexane: ethyl acetate (4: methanol: (5: v/v). Lieberman Burchard reagent. result shows that contains compounds. Quantitatively, secondary metabolites were soluble solvent more than solvent. However, qualitatively, dissolve solvents have varied Rf value terpenoids, especially v/v) various