Determination of Glucose by an Improved Enzymatic Procedure
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چکیده
منابع مشابه
Determination of glucose by an improved enzymatic procedure.
The glucoseoxidase-peroxidasesystem for the enzymatic determination of glucose was investigated with respect to the instability of the enzyme reagent, the buffering capacity of the reaction milieu, and the stability and sensitivity of the final chromogen. An improved procedure was developed in which the enzyme solution is both stabilized and more adequately buffered by reconstitution in 40% aqu...
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An automated procedure for the enzymatic determination of “true” glucose with the glucose oxidase-peroxidase system is described. A threefold increase in sensitivity was obtained through meaurement of the stable, pink chromogen obtained at high acid concentration. Good reproducibility and stoichiometry was obtained over the range of 50-400 mg./l00 ml. at a sampling rate of 40/hr. The normal ran...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1961
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/7.5.542