Development and Leading-Edge Application of Innovative Photoaffinity Labeling
نویسندگان
چکیده
منابع مشابه
Development and leading-edge application of innovative photoaffinity labeling.
Photoaffinity labeling has become increasingly important with the development of powerful specific probes that are synthesized by installing a photo-activatable functional group (photophore) on the framework of biological ligands. The present review summarizes the development of diazirine-based photoaffinity labeling by focusing on its application to the structural elucidation of ligand-accepti...
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Photoaffinity labeling is a recently introduced method for covalently binding chemical tags to the active sites of protein molecules, which is potentially capable of very great specificities of labeling. A labeling reagent is used that is converted by photolysis to an extremely reactive intermediate. According to the expected mechanism, the reagent molecules that are specifically and reversibly...
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Photoaffinity labeling is a powerful technique for identifying a target protein. A high degree of labeling specificity can be achieved with this method in comparison to chemical labeling. Human serum albumin (HSA) and α1-acid glycoprotein (AGP) are two plasma proteins that bind a variety of endogenous and exogenous substances. The ligand binding mechanism of these two proteins is complex. Fatty...
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Photoaffinity labeled (3diazoacetoxy)-9-CC-retinal (1) and (9-methylenediazoacetoxy)9-c&retinal (2) were synthesized and bound to bovine opsin to obtain visual pigment analogs having absorption maxima at 465 and 460 nm respectively. Binding studies established that synthetic retinals 1 and 2 bind to the natural binding site and that the integrity of the diazoacetoxy photoaffinity label is prese...
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A photoaffinity label for corticotropin (ACTH) receptors was prepared by selective chemical modification of the single tryptophan residue in the hormone by reaction with 2-nitro-5-azidophenylsulfenyl chloride. The photoreactive derivative, [(2-nitro-5-azidophenylsulfenyl)-Trp9]ACTH (2,5-NAPS-ACTH), stimulated corticosterone synthesis to 60% of the maximal rate induced by ACTH in isolated rat ad...
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ژورنال
عنوان ژورنال: CHEMICAL ^|^ PHARMACEUTICAL BULLETIN
سال: 2015
ISSN: 0009-2363,1347-5223
DOI: 10.1248/cpb.c14-00645