Development and Validation of an Ovine Progressive Pneumonia Virus Quantitative PCR

Development and validation of an ovine progressive pneumonia virus quantitative PCR.

Ovine progressive pneumonia virus (OPPV) infects at least one sheep in 81% of U.S. sheep flocks, as determined by serology, and can cause viral mastitis, arthritis, dyspnea, and cachexia. Diagnostic tests that quantify OPPV proviral load in peripheral blood leukocytes (PBL) provide an additional method for identification of infected sheep and may help to further understanding of the pathogenesi...

development and implementation of an optimized control strategy for induction machine in an electric vehicle

in the area of automotive engineering there is a tendency to more electrification of power train. in this work control of an induction machine for the application of electric vehicle is investigated. through the changing operating point of the machine, adapting the rotor magnetization current seems to be useful to increase the machines efficiency. in the literature there are many approaches wh...

Ovine Progressive Pneumonia: A Brief Overview

Validation of Quantitative PCR Assays

can assess the safety of biotechnology products. Q-PCR precisely quantifies the amount of the nucleic acid target sequence in DNA or RNA extracted from a variety of samples including animal tissues, final products, cell banks, chromatography eluates, and bulk harvest material. During amplification with target-specific oligonucleotide primers, a fluorogenic oligonucleotide probe — with both repo...

Surface envelope glycoprotein is B-lymphocyte immunodominant in sheep naturally infected with ovine progressive pneumonia virus.

The B-lymphocyte-immunodominant antigen involved in naturally ovine progressive pneumonia virus (OPPV)-infected mature sheep remains unknown. Therefore, the amount of antibody in sera from 10 naturally OPPV-infected sheep was evaluated by immunoprecipitation (IP) of the major viral proteins in [(35)S]methionine/cysteine-labeled OPPV (whole virus) lysate. Using an excess of OPPV proteins in whol...