Development and Validation of Reverse-phase High-performance Liquid Chromatography Method for Novel Synthetic Pyridine Derivative

Development and validation of stability indicating method for the determination of exemestane by reverse phase high performance liquid chromatography.

Exemestane is an aromatase inhibitor used in the treatment of breast cancer. A selective stability-indicating reversed-phase high performance liquid chromatography (RP-HPLC) method has been developed which can separate and accurately quantitate low levels of exemestane. The stability-indicating capability of the method was demonstrated by adequate separation of exemestane and all the degradatio...

A Novel High-Performance Liquid Chromatography Method for Detection of Alginate in Pseudomonas aeruginosa

Background and Objective: The opportunistic pathogen Pseudomonas aeruginosa secrets a capsule-like polysaccharide called alginate which is important for evasion of host defenses, especially in patients with suppressed immunity. Method of alginate determination has an important role in the study of microb...

development and validation of high performance liquid chromatography for determination of nystatin in pharmaceutical formulations

A Novel Reverse Phase High Performance Liquid Chromatography Method For Enantiomeric Separation of Pregabalin With Immobilized Zwitterionic Stationary Phase

Background: The objective of present investigation was to develop simple, sensitive, specific, precise and accurate reversed phase high performance liquid chromatographic (RP-HPLC) method and subsequent validation of the method for the determination of R-isomer in Pregabalin. Method: The chromatographic method was carried out using Chiral pak ZWIX (+) column (250mm x 4.0mm i.d., 3μm particle si...

Development and Validation of A Reverse-Phase High Performance Liquid Chromatography Analysis for Paracetamol in Tablets Formulations

A simple yet sensitive high performance liquid chromatography method was developed and validated for the determination of paracetamol (PCM) content in different tablets dosage form. The mobile phase consisted of a mixture of methanol: water (80:20 v/v). The HPLC analysis was performed at a flow rate of 1 mL/min using a Thermo Synchronise C18 (150 × 4.6mm, 5μM) and an UV detection wavelength of ...