Development of a loop-mediated isothermal amplification and PCR assays for rapid and simple detection of Campylobacter fetus subsp. venerealis
نویسندگان
چکیده
منابع مشابه
Rapid detection of infectious bursal disease by loop-mediated isothermal amplification for field analysis
Infectious bursal disease (IBD) is an immunosuppressive, acute and highly contagious illness of growing-poultry stock infected with infectious bursal disease virus (IBDV). It is common in Pakistan, causing potential economic losses throughout the year. The objective of the study is to propose a rapid, sensitive and specific diagnostic tool, and compare it with existing commonly used reverse tra...
متن کاملDevelopment of Nested PCR, Multiplex PCR, and Loop-Mediated Isothermal Amplification Assays for Rapid Detection of Cylindrocladium scoparium on Eucalyptus
Eucalyptus dieback disease, caused by Cylindrocladium scoparium, has occurred in last few years in large Eucalyptus planting areas in China and other countries. Rapid, simple, and reliable diagnostic techniques are desired for the early detection of Eucalyptus dieback of C. scoparium prior to formulation of efficient control plan. For this purpose, three PCR-based methods of nested PCR, multipl...
متن کاملdevelopment of a loop-mediated isothermal amplification assay for rapid detection of yersinia enterocolitica via targeting a conserved locus
background and objectives: loop-mediated isothermal amplification is a novel nucleic acid amplification assay providing as a simple diagnostic tool for rapid identification of microbial diseases in developing countries. in this study, a lamp assay was established for yersinia enterocolitica , a leading cause of acute enterocolitis in young children. materials and methods: lamp assay was establi...
متن کاملdevelopment and diagnostic evaluation of loop-mediated isothermal amplification using a new gene target for rapid detection of helicobacter pylori
conclusions the analytical sensitivity of lamp and pcr methods, using the designed primers, was 8 times more than any other reported methods. the designed methods are specific and sensitive for detection of h. pylori in different clinical and environmental samples. results the detection limits of the lamp and pcr assay were similar and were 10 fg of pure dna of h. pylori, which is equal to 6 co...
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ژورنال
عنوان ژورنال: Microbiology and Immunology
سال: 2010
ISSN: 0385-5600,1348-0421
DOI: 10.1111/j.1348-0421.2010.00233.x