Development of new photon-counting detectors for single-molecule fluorescence microscopy

Development of new photon-counting detectors for single-molecule fluorescence microscopy.

Two optical configurations are commonly used in single-molecule fluorescence microscopy: point-like excitation and detection to study freely diffusing molecules, and wide field illumination and detection to study surface immobilized or slowly diffusing molecules. Both approaches have common features, but also differ in significant aspects. In particular, they use different detectors, which shar...

Supporting Information for “Development of new photon-counting detectors for single-molecule fluorescence microscopy” Supporting Information for “Development of new photon-counting detectors for single-molecule fluorescence microscopy” by X. Michalet

SBR does not depend on the measurement duration, whereas SNR increases with the measurement duration and SBR. These are well known results and do not need further discussion (1-2). However, these simple expressions also show an interesting connection between signal count rate (s) and background count rate (b) illustrated in Fig. S6. Fig. S6 shows curves of constant SNR at a given SBR, as a func...

New photon-counting detectors for single-molecule fluorescence spectroscopy and imaging.

Solution-based single-molecule fluorescence spectroscopy is a powerful new experimental approach with applications in all fields of natural sciences. Two typical geometries can be used for these experiments: point-like and widefield excitation and detection. In point-like geometries, the basic concept is to excite and collect light from a very small volume (typically femtoliter) and work in a c...

Photon-HDF5: an open file format for single-molecule fluorescence experiments using photon-counting detectors

We introduce Photon-HDF5, an open and efficient file format to simplify exchange and long term accessibility of data from single-molecule fluorescence experiments based on photon-counting detectors such as single-photon avalanche diode (SPAD), photomultiplier tube (PMT) or arrays of such detectors. The format is based on HDF5, a widely used platformand language-independent hierarchical file for...

Single Photon Counting Detectors for TCSPC