Development of Real-Time Reverse Transcriptase PCR Assays To Detect and Serotype Dengue Viruses
نویسندگان
چکیده
منابع مشابه
Development of real-time reverse transcriptase PCR assays to detect and serotype dengue viruses.
Serotyping dengue virus (DENV) from suspect human specimens is crucial for developing sound epidemiological control measurements early in the transmission season and for effective patient management. We modified DENV consensus D1 (mD1) and serotype-specific TS2 (mTS2) and redesigned serotype-specific TS1 (rTS1) and TS4 (rTS4) as described previously in the conventional capsid and premembrane ge...
متن کاملDevelopment and evaluation of serotype- and group-specific fluorogenic reverse transcriptase PCR (TaqMan) assays for dengue virus.
Five fluorogenic probe hydrolysis (TaqMan) reverse transcriptase PCR (RT-PCR) assays were developed for serotypes 1 to 4 and group-specific detection of dengue virus. Serotype- and group-specific oligonucleotide primers and fluorogenic probes were designed against conserved regions of the dengue virus genome. The RT-PCR assay is a rapid single-tube method consisting of a 30-min RT step linked t...
متن کاملDevelopment of real-time reverse transcriptase PCR assays for the detection of Punta Toro
29 30 Background: Research with high biocontainment pathogens such as Rift Valley fever virus 31 (RVFV) and Lassa virus (LASV) is expensive, potentially hazardous, and limited to select 32 institutions. Surrogate pathogens such as Punta Toro virus (PTV) for RVFV infection and 33 Pichinde virus (PICV) for LASV infection allow research to be performed under more 34 permissive BSL-2 conditions. Al...
متن کاملDevelopment of a sensitive real-time reverse transcriptase PCR assay with an internal control to detect and quantify chikungunya virus.
BACKGROUND The chikungunya virus (CHIKV; Alphavirus, Togaviridae) has emerged in the south Western Indian Ocean since early 2005. A major outbreak of CHIKV infection occurred in Réunion Island, where the virus is transmitted by Aedes albopictus mosquitoes. Facing an outbreak of unprecedented magnitude, we developed a rapid, sensitive, and reliable assay for the detection and quantification of C...
متن کاملDevelopment and evaluation of fluorogenic TaqMan reverse transcriptase PCR assays for detection of dengue virus types 1 to 4.
The fluorogenic TaqMan reverse transcriptase PCR (RT-PCR) assay was developed for detecting each of the dengue virus (DV) types 1 to 4. DV genome was detected in all the 35 serum samples from confirmed dengue cases by the TaqMan RT-PCR, although it was not detected in 13 and 21% by conventional type-specific and cross-reactive RT-PCR, respectively.
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2006
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.44.4.1295-1304.2006