Diagnosis of Acanthamoeba keratitis in Mashhad, Northeastern Iran: A Gene-Based PCR Assay
نویسندگان
چکیده
منابع مشابه
Use of 18S rRNA gene-based PCR assay for diagnosis of acanthamoeba keratitis in non-contact lens wearers in India.
Identification of Acanthamoeba cysts and trophozoites in ocular tissues requires considerable expertise and is often time-consuming. An 18S rRNA gene-based PCR test, highly specific for the genus Acanthamoeba, has recently been reported in the molecular diagnosis of Acanthamoeba keratitis. This PCR assay was compared with conventional microbiological tests for the diagnosis of Acanthamoeba kera...
متن کاملGenotyping of Acanthamoeba Species Isolated from Keratitis Patients by PCR Sequencing Methods in Tehran, Iran
Background and Aims: Amoebae of the genus Acanthamoeba are unicellular amphizoic opportunistic pathogens that may cause fatal granulomatous encephalitis, eye keratitis, amebic pneumonitis and skin nodules as well as abscesses in humans and animals. Acanthamoeba keratitis is caused by trauma to the eye, contaminated cleaning solutions and the use of contact lenses. The aim of the present study w...
متن کاملcomparison of a pcr-based method with culture and direct examination for diagnosis of acanthamoeba keratitis
background: the aim was to compare three different methods (direct examination, culture and pcr methods) for the diagnosis of acanthamoeba keratitis (ak) in corneal scrapes. methods: twenty eight corneal scrapes and contact lenses were collected from keratitis patients and referred to the department of medical parasitology and mycology, school of public health, tehran university of medical ...
متن کاملAcanthamoeba keratitis--importance of the early diagnosis.
This is a case report of Acanthamoeba as a causative agent of keratitis at the Department of Ophthalmology, University Hospital Rijeka. Delay in treatment led to an advanced stage of the disease with multiple complications. Initially, presented symptoms were similar to those of herpetic keratitis. In the mean time progression of the disease led to a cloudy cornea with a stromal ring infiltrate,...
متن کاملDiagnosis and management of Acanthamoeba keratitis
Purpose of review This paper reviews the literature generated on Acanthamoeba keratitis since 1998. Recent findings Acanthamoeba infections may be on the rise. Contact lenses are the biggest risk factor for their development. Silicone hydrogel lenses are increasingly prescribed and may be ‘more sticky’ to Acanthamoeba organisms. Orthokeratology for the treatment of myopia has been associated wi...
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ژورنال
عنوان ژورنال: Iranian Journal of Parasitology
سال: 2021
ISSN: 1735-7020
DOI: 10.18502/ijpa.v16i1.5530