Diagnostic applications for Lassa fever in limited-resource settings

A Diagnostic Scoring Model for Leptospirosis in Resource Limited Settings

BACKGROUND Leptospirosis is a zoonotic infection with significant morbidity and mortality. The clinical presentation of leptospirosis is known to mimic the clinical profile of other prevalent tropical fevers. Laboratory confirmation of leptospirosis is based on the reference standard microscopic agglutination test (MAT), direct demonstration of the organism, and isolation by culture and DNA det...

Resuscitation in resource-limited settings.

Intrapartum-related hypoxia leading to deaths and disabilities continues to be a global challenge, especially in resource-limited settings. Primary prevention during labour is likely to have a significant impact, but secondary prevention with focus on immediate basic stabilization at birth can effectively reduce a large proportion of these adverse outcomes as demonstrated in the resource-rich s...

Development of recombinant nucleoprotein-based diagnostic systems for Lassa fever.

Diagnostic systems for Lassa fever (LF), a viral hemorrhagic fever caused by Lassa virus (LASV), such as enzyme immunoassays for the detection of LASV antibodies and LASV antigens, were developed using the recombinant nucleoprotein (rNP) of LASV (LASV-rNP). The LASV-rNP was expressed in a recombinant baculovirus system. LASV-rNP was used as an antigen in the detection of LASV-antibodies and as ...

Analytic Biosurveillance Methods for Resource-Limited Settings

Background The 21st century has seen advances in many aspects of global disease surveillance.1 These advances have been driven by heightened concerns over perceived threats to public health both from natural pathogens and from bioterrorism. These concerns have led to mandated improvements at the international level, through revision of International Health Regulations of the World Health Organi...

Viral load testing in resource-limited settings.