Differences between cultured cortical neurons by trypsin and papain digestion

Studies by Trypsin Digestion in Vesicle Membranes

Purified adrenocortical microsomal P-450~21 was incorporated into vesicle membranes composed of phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine a t a molar ratio of 5 3 : 1. Trypsinolysis of the incorporated P 4 5 0 ~ ~ ~ resulted in the formation of 30-, 2 5 , and 20-kDa fragments. Similar fragment formation was observed by trypsinolysis of bovine adrenocortical microsome...

Kinetics of Trypsin Digestion

The anomalies of enzyme action have frequently been accounted for by the assumption of an intermediate compound between the enzyme and the substrate. I t was shown, however, by Nelson and Hitchcock t that in the case of invertase the experiments could not be accurately calculated on this basis. The writer found with trypsin' that although the effect of varying the substrate concentration could ...

Induction of Synapses by Agrin in Cultured Cortical Neurons

Expression of CXCL10 in cultured cortical neurons.

Chemokines expressed in neurons are important mediators in neuron-neuron and neuron-glia signaling. One of these chemokines is CCL21 that activates microglia via the chemokine receptor CXCR3. As neurons also express CXCL10, a main ligand for CXCR3, we have thus investigated in detail the expression pattern of CXCL10 in neurons. We show that CXCL10 is constitutively expressed by neurons, is stor...

Dissociated Neurons and Glial Cells Derived from Rat Inferior Colliculi after Digestion with Papain

The formation of gliosis around implant electrodes for deep brain stimulation impairs electrode-tissue interaction. Unspecific growth of glial tissue around the electrodes can be hindered by altering physicochemical material properties. However, in vitro screening of neural tissue-material interaction requires an adequate cell culture system. No adequate model for cells dissociated from the inf...