Differentiable model-based adaptive optics for two-photon microscopy

Aberrations limit scanning fluorescence microscopy when imaging in scattering materials such as biological tissue. Model-based approaches for adaptive optics take advantage of a computational model the optical setup. Such models can be combined with optimization techniques machine learning frameworks to find aberration corrections, was demonstrated focusing laser beam through aberrations onto camera [ Opt. Express 28 26436 ( ) 10.1364/OE.403487 ]. Here, we extend this approach two-photon microscopy. The developed sensorless technique finds corrections samples and will useful range application, example brain