Differential Transcription of Bacteriophage φX174 Genes at 37°C and 42°C
نویسندگان
چکیده
منابع مشابه
Differential Transcription of Bacteriophage φX174 Genes at 37°C and 42°C
To investigate how high temperature affects viral transcription, the absolute amounts of mRNA for six bacteriophage φX174 genes were compared at 37 °C and 42 °C using Q-PCR. At 37 °C, mRNA levels for all genes were consistent with previous studies, but at 42 °C mRNA levels for four genes were significantly different from levels at 37 °C. Transcript levels were higher for genes B and D; the prom...
متن کاملComparative proteomic analysis of Campylobacter jejuni cultured at 37C and 42C.
Campylobacter jejuni has the potential to thrive at 37C (e.g., in the human intestinal tract) and 42C (e.g., in the poultry intestinal tract). We aimed to determine the protein expression profiles of C. jejuni cultured at 37C and 42C in vitro by two-dimensional electrophoresis (2DE). The differentially expressed spots/proteins between C. jejuni cultured at 37C and 42C were defined when their ex...
متن کاملTranscription and translation of prereplicative bacteriophage T4 genes in vitro.
Bacteriophage T4 DNA was used to direct transcription and translation in vitro in extracts prepared from uninfected Escherichia coli. The radioactive protein products of the cell-free reactions were examined on sodium dodecyl sulfate acrylamide gels. We conclude that the cell-free system prepared from uninfected E. coli has the capacity to synthesize most T4 prereplicative RNAs and proteins. Fu...
متن کاملSelection Affects Genes Involved in Replication during Long-Term Evolution in Experimental Populations of the Bacteriophage φX174
Observing organisms that evolve in response to strong selection over very short time scales allows the determination of the molecular mechanisms underlying adaptation. Although dissecting these molecular mechanisms is expensive and time-consuming, general patterns can be detected from repeated experiments, illuminating the biological processes involved in evolutionary adaptation. The bacterioph...
متن کاملMycobacterium avium subsp. paratuberculosis induces differential cytosine methylation at miR-21 transcription start site region
Mycobacterium aviumsubspecies paratuberculosis (MAP), as an obligate intracellular bacterium, causes paratuberculosis (Johne’s disease) in ruminants. Plus, MAP has consistently been isolated from Crohn’s disease (CD) lesions in humans; a notion implying possible direct causative ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: PLoS ONE
سال: 2012
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0035909