Diffusional restrictions in glyoxyl-agarose immobilized penicillin G acylase of different particle size and protein loading
نویسندگان
چکیده
منابع مشابه
Diffusional restrictions in glyoxyl-agarose immobilized penicillin G acylase of different particle size and protein loading
Particle size and enzyme protein loading are design parameters of enzyme immobilization affecting biocatalyst performance that can be varied within broad margins. Their effect on mass transfer limitations at different bulk penicillin G concentrations has been studied with glyoxyl agarose immobilized penicillin G acylase biocatalysts of average particle size of 5·10m and 10·10m at protein loadin...
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BACKGROUND Immobilized Penicillin G Acylase (PGA) derivatives are biocatalysts that are industrially used for the hydrolysis of Penicillin G by fermentation and for the kinetically controlled synthesis of semi-synthetic beta-lactam antibiotics. One of the most used supports for immobilization is glyoxyl-activated agarose, which binds the protein by reacting through its superficial Lys residues....
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The synthesis of amoxicillin with immobilized penicillin G acylase (PGA) in aqueous medium was investigated. The parameters studied were: time course of amoxicillin production, concentration of substrates: hydroxyphenylglycine methyl ester (HPGM) and 6-aminopeicillanic acid (6APA) and the e ect of enzyme (PGA) content and pH, under variable and constant conditions and temperature variations. In...
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ژورنال
عنوان ژورنال: Electronic Journal of Biotechnology
سال: 2010
ISSN: 0717-3458,0717-3458
DOI: 10.2225/vol13-issue1-fulltext-12