Direct electrochemistry of nitrate reductase from the fungus Neurospora crassa
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چکیده
منابع مشابه
Complex I from the fungus Neurospora crassa.
Respiratory chain complex I is a complicated enzyme of mitochondria, that couples electron transfer from NADH to ubiquinone to the proton translocation across the inner membrane of the organelle. The fungus Neurospora crassa has been used as one of the main model organisms to study this enzyme. Complex I is composed of multiple polypeptide subunits of dual genetic origin and contains several pr...
متن کاملInduction and repression of nitrate reductase in Neurospora crassa.
Synthesis of wild-type Neurospora crassa assimilatory nitrate reductase is induced in the presence of nitrate ions and repressed in the presence of ammonium ions. Effects of several Neurospora mutations on the regulation of this enzyme are shown: (i) the mutants, nit-1 and nit-3, involving separate lesions, lack reduced nicotinamide adenine dinucleotide (NADPH)-nitrate reductase activity and at...
متن کاملNitrogen metabolite repression of nitrate reductase in Neurospora crassa: effect of the gln-1a locus.
Nicotinamide adenine dinucleotide phosphate (reduced form)-nitrate reductase was freed from ammonium repression in a Neurospora crassa mutant having drastically lowered glutamine synthetase activity, gln-1a. The general phenomenon of nitrogen metabolite repression required glutamine or some aspect of glutamine metabolism.
متن کاملInvolvement of a B-type cytochrome in the assimilatory nitrate reductase of Neurospora crassa.
The enzyme systems which catalyze the reduction of nitrate to nitrite have generally been classified into two broad types: (a) assimilatory nitrate reductase, which is responsible for the first step in the reduction of nitrate for the ultimate biosynthesis of nitrogen-containing cell constituents (e.g., amino acids aind purines and pyrimidines), and (b) respiratory or dissimilatory nitrate redu...
متن کاملNitrate transport system in Neurospora crassa.
Nitrate uptake in Neurospora crassa has been investigated under various conditions of nitrogen nutrition by measuring the rate of disappearance of nitrate from the medium and by determining mycelial nitrate accumulation. The nitrate transport system is induced by either nitrate or nitrite, but is not present in mycelia grown on ammonia or Casamino Acids. The appearance of nitrate uptake activit...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Bioenergetics
سال: 2016
ISSN: 0005-2728
DOI: 10.1016/j.bbabio.2016.04.001