Direct PCR amplification from saliva sample using non-direct multiplex STR kits for forensic DNA typing
نویسندگان
چکیده
منابع مشابه
STR DNA typing: increased sensitivity and efficient sample consumption using reduced PCR reaction volumes.
Improvements in detection limits/sensitivity and lower sample consumption are potential benefits of reducing PCR reaction volumes used in forensic DNA typing of crime scene samples. This premise was studied first with experimental mixtures and a nine-loci megaplex, which demonstrated stochiometric amplification and accurate detection. Next, adjudicated casework samples were subjected to amplifi...
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Forensic DNA typing has been a constantly evolving field driven by innovations from academic laboratories as well as kit manufacturers. Central to these technological advances has been the transition from multilocus-probe restriction fragment length polymorphism (RFLP) methods to short tandem repeat (STR) PCR-based assays. STRs are now the markers of choice for forensic DNA typing and a wide va...
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Direct PCR was first used in the field of microbiology, where it was more commonly known as colony PCR. Since then, many more applications of direct PCR has been described in other fields where it has aided in diagnosis of infectious diseases and in botany. Direct PCR is a technique where amplification is carried out on samples without prior extraction, purification or quantification. In forens...
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Pyrosequencing is a highly effective method for quantitatively genotyping short genetic sequences, but it currently is hampered by a labor-intensive sample preparation process designed to isolate single-stranded DNA from double-stranded products generated by conventional PCR. Here linear-after-the-exponential (LATE)-PCR is introduced as an efficient and potentially automatable method of directl...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2021
ISSN: 2045-2322
DOI: 10.1038/s41598-021-86633-0