Directed adenovirus evolution using engineered mutator viral polymerases

Directed adenovirus evolution using engineered mutator viral polymerases

Adenoviruses (Ads) are the most frequently used viruses for oncolytic and gene therapy purposes. Most Ad-based vectors have been generated through rational design. Although this led to significant vector improvements, it is often hampered by an insufficient understanding of Ad's intricate functions and interactions. Here, to evade this issue, we adopted a novel, mutator Ad polymerase-based, 'ac...

DNA and RNA Polymerases with Expanded Substrate Scope : Synthesis of Modified Nucleic Acids Using Engineered Polymerases Generated by Directed Evolution

DNA polymerases engineered by directed evolution to incorporate non-standard nucleotides

DNA polymerases have evolved for billions of years to accept natural nucleoside triphosphate substrates with high fidelity and to exclude closely related structures, such as the analogous ribonucleoside triphosphates. However, polymerases that can accept unnatural nucleoside triphosphates are desired for many applications in biotechnology. The focus of this review is on non-standard nucleotides...

Directed evolution of DNA polymerases for next-generation sequencing.

*Fax: (+1) 858-784-7472, [email protected], Homepage: http://www.scripps.edu/chem/romesberg. Supporting information for this article is available on the WWW under http://www.angewandte.org or from the author. NIH Public Access Author Manuscript Angew Chem Int Ed Engl. Author manuscript; available in PMC 2011 August 9. Published in final edited form as: Angew Chem Int Ed Engl. 2010 August 9; 49(...

Evolution of Tertiary Structure of Viral RNA Dependent Polymerases

Viral RNA dependent polymerases (vRdPs) are present in all RNA viruses; unfortunately, their sequence similarity is too low for phylogenetic studies. Nevertheless, vRdP protein structures are remarkably conserved. In this study, we used the structural similarity of vRdPs to reconstruct their evolutionary history. The major strength of this work is in unifying sequence and structural data into a...