Distinct requirement of RNA polymerase II CTD phosphorylations in budding and fission yeast
نویسندگان
چکیده
منابع مشابه
Punctuation and syntax of the RNA polymerase II CTD code in fission yeast.
The primary structure and phosphorylation pattern of the tandem Y(1)S(2)P(3)T(4)S(5)P(6)S(7) repeats of the RNA polymerase II carboxyl-terminal domain (CTD) convey information about the transcription apparatus--a CTD code--to a large ensemble of CTD-binding receptor proteins. Four of the seven coding "letters" of the fission yeast CTD (Tyr1, Pro3, Ser5, Pro6) are essential in vivo, but the gram...
متن کاملDisengaging polymerase: Terminating RNA polymerase II transcription in budding yeast☆
Termination of transcription by RNA polymerase II requires two distinct processes: The formation of a defined 3' end of the transcribed RNA, as well as the disengagement of RNA polymerase from its DNA template. Both processes are intimately connected and equally pivotal in the process of functional messenger RNA production. However, research in recent years has elaborated how both processes can...
متن کاملTranscriptional termination signals for RNA polymerase II in fission yeast.
Transcription 'run-on' (TRO) analysis using permeabilized yeast cells indicates that transcription terminates between 180 and 380 bp downstream of the poly(A) site of the Schizosaccharomyces pombe ura4 gene. Two signals direct RNA polymerase II (pol II) to stop transcription: the previously identified 3' end formation signals located close to the poly(A) site and an additional downstream elemen...
متن کاملThe RNA polymerase II CTD coordinates transcription and RNA processing.
The C-terminal domain (CTD) of the RNA polymerase II largest subunit consists of multiple heptad repeats (consensus Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7), varying in number from 26 in yeast to 52 in vertebrates. The CTD functions to help couple transcription and processing of the nascent RNA and also plays roles in transcription elongation and termination. The CTD is subject to extensive post-tra...
متن کاملA Gene-Specific Requirement of RNA Polymerase II CTD Phosphorylation for Sexual Differentiation in S. pombe
BACKGROUND The switch from cellular proliferation to differentiation occurs to a large extent through specific programs of gene expression. In fission yeast, the master regulator of sexual differentiation, ste11, is induced by environmental conditions leading to mating and meiosis. RESULTS We show that phosphorylation of serine 2 (S2P) in the C-terminal domain of the largest subunit of the RN...
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ژورنال
عنوان ژورنال: Transcription
سال: 2012
ISSN: 2154-1264,2154-1272
DOI: 10.4161/trns.21066