Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst

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Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst.

The first two lineages to differentiate from a pluripotent cell population during mammalian development are the extraembryonic trophectoderm (TE) and the primitive endoderm (PrE). Whereas the mechanisms of TE specification have been extensively studied, segregation of PrE and the pluripotent epiblast (EPI) has received comparatively little attention. A current model of PrE specification suggest...

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Formation of distinct cell types in the mouse blastocyst.

Early development of the mouse comprises a sequence of cell fate decisions in which cells are guided along a pathway of restricted potential and increasing specialisation. The first choice faced by cells of the embryo is whether to become trophectoderm (TE) or inner cell mass (ICM); TE is an extra-embryonic tissue which will form the embryonic portion of the placenta, whilst ICM gives rise to c...

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Atypical protein kinase C couples cell sorting with primitive endoderm maturation in the mouse blastocyst

During mouse pre-implantation development, extra-embryonic primitive endoderm (PrE) and pluripotent epiblast precursors are specified in the inner cell mass (ICM) of the early blastocyst in a 'salt and pepper' manner, and are subsequently sorted into two distinct layers. Positional cues provided by the blastocyst cavity are thought to be instrumental for cell sorting; however, the sequence of e...

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Oct4 cell-autonomously promotes primitive endoderm development in the mouse blastocyst.

In embryonic stem (ES) cells and in early mouse embryos, the transcription factor Oct4 is an essential regulator of pluripotency. Oct4 transcriptional targets have been described in ES cell lines; however, the molecular mechanisms by which Oct4 regulates establishment of pluripotency in the epiblast (EPI) have not been fully elucidated. Here, we show that neither maternal nor zygotic Oct4 is re...

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P-126: Stem Cell Factor Increases Blastocyst Formation in Mouse Two-Cell Embryo Culture

Background: It is demonstrated that c-Kit( receptor of stem cell factor) mRNA is expressed in late 2-cell stage to the expanded and hatched blastocyst and the stem cell factor (SCF) transcript is detected in the oviduct and uterus. The aim of this study was to investigate the effect of different doses of SCF on mouse 2-cell embryo development in vitro. Materials and Methods: 4-6 weeks old femal...

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ژورنال

عنوان ژورنال: Developmental Biology

سال: 2008

ISSN: 0012-1606

DOI: 10.1016/j.ydbio.2008.05.540