DNA polymerase β: Closing the gap between structure and function

Closing The Gap Between Discourse Structure And Communicative Intention

Structure and Mechanism of DNA Polymerase β

DNA polymerase (pol) β is a small eukaryotic DNA polymerase composed of two domains. Each domain contributes an enzymatic activity (DNA synthesis and deoxyribose phosphate lyase) during the repair of simple base lesions. These domains are termed the polymerase and lyase domains, respectively. Pol β has been an excellent model enzyme for studying the nucleotidyl transferase reaction and substrat...

DNA Polymerase β Gap-Filling Translesion DNA Synthesis

Although the primary function of DNA polymerase (pol) β is associated with gap-filling DNA synthesis as part of the DNA base excision repair pathway, translesion synthesis activity has also been described. To further understand the potential role of pol β-catalyzed translesion DNA synthesis (TLS) and the structure-function relationships of specific residues in pol β, wild-type and selected muta...

Interaction between DNA Polymerase β and BRCA1

The breast cancer 1 (BRCA1) protein is a tumor suppressor playing roles in DNA repair and cell cycle regulation. Studies of DNA repair functions of BRCA1 have focused on double-strand break (DSB) repair pathways and have recently included base excision repair (BER). However, the function of BRCA1 in BER is not well defined. Here, we examined a BRCA1 role in BER, first in relation to alkylating ...

Closing the Gap Between Object - OrientedModeling of Structure

Due to hardware developments, strong application needs and the overwhelming innuence of the net in almost all areas, distributed systems, especially software systems, have become one of the most important topics for nowadays software industry. Unfortunately, distribution adds its share to the problems of developing complex software systems. Heterogeneity in both, hardware and software, concurre...