DNA restriction dependent on two recognition sites: activities of the Sfi I restriction-modification system in Escherichia coli
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چکیده
منابع مشابه
Cloning and expression of the Pst I restriction-modification system in Escherichia coli.
Here we report the cloning and preliminary characterization of the Pst I restriction-modification system of Providencia stuartii 164. Transformants of Escherichia coli carrying the Pst I gene system inserted into the cloning vector pBR322 were selected on the basis of acquired resistance to bacteriophage lambda infection. Pst I endonuclease was detected in osmotic shock fluid from each of the r...
متن کاملTwo-step cloning and expression in Escherichia coli of the DNA restriction-modification system StyLTI of Salmonella typhimurium.
The StyLTI restriction-modification system is common to most strains of the genus Salmonella, including Salmonella typhimurium. We report here the two-step cloning of the genes controlling the StyLTI system. The StyLTI methylase gene (mod) was cloned first. Then, the companion endonuclease gene (res) was introduced on a compatible vector. A strain of S. typhimurium sensitive to the coliphage la...
متن کاملAlignment of Sfi I sites with the Not I restriction map of Schizosaccharomyces pombe genome.
A Sfi I restriction map of the fission yeast Schizosaccharomyces pombe genome was aligned with the Not I restriction map. There are 16 Sfi I sites in the S. pombe genome. Three Sfi I sites are on chromosome III which is devoid of Not I sites. The sizes of the entire genome and individual chromosomes, calculated from the Sfi I fragment sizes, are consistent with that calculated from the Not I fr...
متن کاملLack of regulation of the modification-dependent restriction enzyme McrBC in Escherichia coli.
Restriction alleviation (RA) by the type I restriction enzyme EcoKI is caused by treatments that damage DNA. RA is due to proteolysis of the EcoKI HsdR subunit by the ClpXP ATP-dependent protease. Here we show that the modification-dependent enzyme McrBC is not subject to RA, although it is moderately sensitive to ClpAP.
متن کاملThe average spacing of restriction enzyme recognition sites in DNA.
The discovery of naturally occurring enzymes which cleave DNA at sites specific to particular nucleotide sequences has had a great impact on molecular biology. The function of these enzymes in uivo is to protect bacterial cells from viral invasion by degradation of foreign DNA. Several hundred of these “restriction” enzymes are known and they are a very common tool both for analysis and manipul...
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ژورنال
عنوان ژورنال: Molecular Microbiology
سال: 1999
ISSN: 0950-382X,1365-2958
DOI: 10.1046/j.1365-2958.1999.01266.x