DNA restriction site mapping of adenovirus type 16 withBamI,EcoRI,HpaI ANDSalI
نویسندگان
چکیده
منابع مشابه
Isolation of DNA from agarose gels using DEAE-paper. Application to restriction site mapping of adenovirus type 16 DNA.
A new method for isolating DNA from agarose gels is described. The method involves the simultaneous transfer of all DNA-fragments from an agarose slab gel onto DEAE-cellulose paper and the elution of the individual fragments from the paper with 1 M NaCl. DNA isolated from agarose gels in this way is susceptible to cleavage with several restriction endonucleases, and can be labeled in vitro with...
متن کاملAlgorithms for the restriction-site mapping of DNA molecules.
Two algorithms are described that rapidly construct restriction maps of DNA molecules from single- and double-digest data. The implementation of these algorithms into the Pascal programs AMAPK and AMARD allows such mapping to be done on microcomputers.
متن کاملPhylogenetics in Clarkia (Onagraceae): Restriction Site Mapping of Chloroplast DNA
Restriction site maps of chloroplast DNA (cpDNA), maternally inherited in Clarkia, were obtained in representative species of all seven diploid sections, the allotetraploid sect. Clarkia, and the outgroup Epilobium brachycarpum. CpDNA restriction site variation was analyzed by Wagner and Dollo parsimony, bootstrapping, and genetic distance methods. Four shortest Wagner trees, one of which was a...
متن کاملRestriction site mapping is in separation theory
A computer algorithm for restriction-site mapping consists of a generator of partial maps and a consistency checker. This paper examines consistency checking and argues that a method based on separation theory extracts the maximum amount of information from fragment lengths in digest data. It results in the minimum number of false maps being generated.
متن کاملFast Approximate Matching in Restriction Site Mapping
This paper presents a trie data structure for fast approximate matching of DNA fragments in a large scale restriction mapping project. It analyzes several parameters that aaect the performance of the data structure and brieey explores strategies on how it might be used and how to proceed in the initial stages of potential algorithms for mapping. The paper then reports on experimental results an...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 1977
ISSN: 0014-5793
DOI: 10.1016/0014-5793(77)80142-7