Dynamic Ratiometric Imaging of Cytosolic Free Ca2+ in Skeletal Muscle Cells Using 340/385-nm Light-Emitting Diode Illuminators
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چکیده
منابع مشابه
A 340/380 nm light‐emitting diode illuminator for Fura‐2 AM ratiometric Ca2+ imaging of live cells with better than 5 nM precision
We report the first demonstration of a fast wavelength-switchable 340/380 nm light-emitting diode (LED) illuminator for Fura-2 ratiometric Ca2+ imaging of live cells. The LEDs closely match the excitation peaks of bound and free Fura-2 and enables the precise detection of cytosolic Ca2+ concentrations, which is only limited by the Ca2+ response of Fura-2. Using this illuminator, we have shown t...
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ژورنال
عنوان ژورنال: IEEE Photonics Journal
سال: 2018
ISSN: 1943-0655,1943-0647
DOI: 10.1109/jphot.2018.2882503