Dynamic response of micropipettes during piezo-assisted intracytoplasmic sperm injection

Piezo-actuated Mouse ICSI (Intracytoplasmic sperm injection)

Intracytoplasmic sperm injection (ICSI) is not only used in human assisted reproduction (ART), but it is also widely employed for veterinary in vitro fertilization during rare species preservation, germ-line rescue of transgenic animals or for any veterinary assisted conception. Piezo-assisted ICSI, which was first described in 1995 [1], can be employed for assisted conception in animals in whi...

Improved material point method for simulating the zona failure response in piezo-assisted intracytoplasmic sperm injection

The material point method (MPM), which is an extension from computational fluid dynamics (CFD) to computational solid dynamics (CSD), is improved for the coupled CFD and CSD simulation of the zona failure response in piezo-assisted intracytoplasmic sperm injection (piezo-ICSI). To evaluate the stresses at any zona material point, a plane stress assumption is made in the local tangent plane of t...

Comparison of Embryo Development between Intracytoplasmic and Piezo-Assisted Sperm Injection after Treating Mouse Sperms by Ca2+ Ionophore

The purpose of this study was to evaluate the efficiency of intracytoplasmic sperm injection (ICSI) and Piezo-assisted sperm injection after pretreatment with calcium ionophore (CaI) on the mouse embryo development. Methods: In this study, the conventional ICSI and Piezo-ICSI procedures were used. The efficacy of the methods was examined after mouse matured oocytes were fertilized with or witho...

Intracytoplasmic sperm injection (ICSI).

Intracytoplasmic Sperm Injection

Intracytoplasmic Sperm Injection (ICSI) is an assisted reproductive technique [4] (ART) initially developed by Dr. Gianpiero D. Palermo [5] in 1993 to treat male infertility [6]. It is most commonly used in conjunction with in vitro [7] fertilization [8] (IVF) or a less commonly used technique called zygote intrafallopian transfer [9] (ZIFT). In natural fertilization [8], the sperm [10] must pe...