Early IgE production is associated with accumulation of CD11b+ classical dendritic cells and CD11b+CD11c- macrophages in subcutaneous adipose tissue

In view of increased incidence IgE-mediated diseases in Russia and worldwide, the efforts various research groups are focused on studying mechanisms that trigger process switching B-lymphocytes to IgE synthesis upon human exposure harmless allergens, including role antigen-presenting cells (APCs) this process. However, distinct APCs long-term penetration low antigen doses via tissue barriers, is yet poorly understood, as well specific features these events entrance through subcutaneous adipose which contains tissueassociated lymphoid clusters (TALC).The aim work was determine relationship between local accumulation development early production a clinically relevant experimental model allergy with administration allergen doses. model, induced, minimal concomitant IgG production, thus mimicking situation observed patients sound allergies. BALB/c mice were immunized for 4 weeks subcutaneously withers area or intraperitoneally (100 ng) high (10 μg) (ovalbumin). Blood samples taken weekly from ELISA testing, antibodies. Provocation tests performed dose allergen, site injection flow cytometric assays, order evaluate contents APC subpopulations. Specific induced mainly by at into withers. When using regimen, we classical CD11b + site, but not peritoneal tissue, absence - classical, inflammatory plasmacytoid, dendritic cells. These findings coincided time days +14 +21. Accumulation CD11c macrophages their CD206 M2 subpopulations terms (days +7 +21) also only after area. The high-dose mediated 1 greater extent than IgE, led earlier (on day 7 th ), macrophage later stages (day 21 ). Thus, start low-dose may be associated its presentation presence macrophages.