Ectopic p21WAF1 Expression Induces Differentiation-specific Cell Cycle Changes in PC12 Cells Characteristic of Nerve Growth Factor Treatment
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چکیده
منابع مشابه
Nerve growth factor-induced differentiation of PC12 cells: evaluation of changes in RNA and DNA metabolism.
The relationship between both DNA and RNA metabolism and the nerve growth factor (betaNGF)-induced differentiation of PC12 cells was investigated. Cells remained in log phase growth for 4 days after exposure to betaNGF. DNA synthesis was similarly unaffected by betaNGF during this time. Thereafter, a gradual decrease in DNA synthesis was observed. In contrast, as many as 40% of the cells displa...
متن کاملNerve Growth Factor–specific Regulation of Protein Methylation during Neuronal Differentiation of PC12 Cells
Protein methylation is a posttranslational modification that can potentially regulate signal transduction pathways in a similar manner as protein phosphorylation. The role of protein methylation in NGF signaling was examined by metabolic labeling of PC12 cell proteins with L-[methyl-3H]methionine and by in vitro labeling of cell proteins with L-[methyl-3H]S-adenosylmethionine. Effects of NGF we...
متن کاملNerve growth factor-induced differentiation changes the cellular organization of regulated Peptide release by PC12 cells.
PC12 cells, like endocrine chromaffin cells, undergo neuronal-like differentiation in response to nerve growth factor (NGF). Here we report that this phenotype conversion produces major changes in release of a green fluorescent protein-tagged neuropeptide-hormone. First, the spatial distribution of the releasable pool is altered; peptide release from untreated cells is supported predominantly b...
متن کاملThe ras suppressor, RSU-1, enhances nerve growth factor-induced differentiation of PC12 cells and induces p21CIP expression.
The Rsu-1 Ras suppressor gene was isolated based on its ability to inhibit v-Ras transformation. Using Rsu-1 transfectants of the pheochromocytoma cell line PC12, we demonstrated previously that Rsu-1 expression inhibited Jun kinase activation but enhanced Erk2 activation in response to epidermal growth factor. In the present study, the Rsu-1 PC12 transfectants were used to investigate the role...
متن کاملNerve growth factor enhances expression of neuron-glia cell adhesion molecule in PC12 cells
The neuron-glia cell adhesion molecule (Ng-CAM) has been identified in mammalian brain tissue and PC12 pheochromocytoma cells as Mr 200,000 and Mr 230,000 species, respectively. When PC12 cells were treated with nerve growth factor (NGF), the amount of Ng-CAM at the cell surface was increased approximately threefold, whereas the amount of the neural cell adhesion molecule (N-CAM) remained uncha...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1998
ISSN: 0021-9258
DOI: 10.1074/jbc.273.36.23517