Effect of a Simvastatin-Impregnated Chitosan Scaffold on Cell Growth and Osteoblastic Differentiation

This study aims to evaluate the effect of chitosan (CS) scaffold, alone, and potential synergistic when impregnated with simvastatin (SIM), on immortalized human bone-marrow mesenchymal stem cells (hbMMSCs) compared CollaCote (CL). CS scaffolds were fabricated seeded hBMMSCs. Samples divided into control groups (negative no added material positive CL added) four experimental groups: CS/SIM 0.01, 0.03, 0.05 mg, respectively. Cell viability, osteoblastic differentiation calcium deposition investigated via AlamarBlue, alkaline phosphate activity assays alizarin red S staining at 1 14 days, At day one, significant difference was noted between regarding cell viability. However, all showed cutback in proliferation alone (p < 0.001). All supported difference. Alkaline increased both time periods mg group other SIM groups, among groups. Chitosan scaffold is a bioactive compatible capable regenerative hBMMSCs promising be used for perforation repair.